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Okti Nadia Poetri
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
PRODUKSI ANTIBODI KUNING TELUR (IgY) ANTI STREPTOCOCCUS MUTANS SEBAGAI ANTI KARIES GIGI Okti Nadia Poetri; Retno D Soejoedono
Jurnal Ilmu Pertanian Indonesia Vol. 11 No. 3 (2006): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The aim of this study was to explore IgY anti Streptococcus mutan production and the ability of Igy Streptococcus mutans blocking adhesion process. The eggs was collected from Single Comb Brown Leghorn which have been immunized by S. mutan. Agar gel precipitation test was done to detect IgY anti S. mutans in serum and egg. Egg which Countain IgY anti S. mutans was collected. IgY anti S. mutans extracted from egg yolk by mean s PEG-Amonium sulfat and purified using fast protein liquid chromatography. The purity of Igy anti S. mutans was determined by UV spectropometer. Biological activities of Igy anti S. mutans to inhibit adhession process was learned by anti adhesion test. We use two dose of IgY, which is 100 ug and 500 ug. Igy anti S.  mutans formen in serum  five weeks after the first immunization while it formed in egg nine weeks after the first immunization. Igy anti S. mutanss still present in serum andegg until twelve weeks from the first immunization. Igy anti S. mutanss  could decrease the amount of bacteria which attach the epithelial cell surface. The amount of sticky bacteria on epithelial cell (without IgY) are 40 cell bacteria/epithelial cell. After blocked by IgY anti S. mutanss  the amount of bacteria turn into 30 cell bacteria/epithelial cell (for dose of 100 ug IgY) and 28 cell bacteria/epitheelial cell (for dose of 500 ug IgY). This research concluded that hens were capable producing IgY anti S. mutanss in egg yolk and it can be used to solve dental caries problem which caused by S. mutanss.
Co-Authors Agustin Indrawati Anindita Sista Widyadhari Anindita Sista Widyadhari Asep Rakhmat Atsmarina Widyadhari Cece Sumantri Chaerul Basri Cut Desna Apriana Dinda Reisinta Dwi Desmiyeni Putri Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Hadri Latif I Wayan T. Wibawan I wayan Teguh Wibawan Koekoeh Santoso Kris Damar Sasi La Jumadin La Jumadin M. Gumilang Pramuwidyatama Melani Wahyu Adiningsih Ni Luh Putu Ika Mayasari Novera Nirmalasanti R. Dikky Indrawan Rahmat Setya Adji Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Rifky Rizkiantino sri murtini . Suwarny Ruhi Trioso Purnawarman Wyanda Arnafia